As the 16S profiles with the gut appear extremely diverse for intracellular and extracellular DNA [33], qPCR is predicted to mirror the NGS-specific community construction equally quantitatively and qualitatively extra intently than move cytometry.
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qPCR-primarily based quantitative microbiome profiling is applicable also for samples made up of a substantial number of host or non-bacterial DNA, during which bacterial density can not be reliably approximated by full DNA produce [five].
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Below, we explain how quantitative PCR (qPCR) performed in parallel to NGS library planning gives an accurate estimation of complete taxon abundances from NGS knowledge and for this reason delivers an attainable Resolution to compositionality in substantial-throughput microbiome analyses. The advantages and possible troubles of the strategy can also be reviewed.
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